Liposome-Based Chemical Barcodes for Single Molecule DNA Detection Using Imaging Mass Spectrometry
Journal article, 2010

We report on a mass-spectrometry (time-of-flight secondary ion mass spectrometry, TOF-SIMS) based method For multiplexed DNA detection utilizing a random array, where the lipid composition of small unilamellar liposomes act as chemical barcodes to identify unique DNA target sequences down to the single molecule level. In a sandwich format, suspended target-DNA to be detected mediates the binding of capture-DNA modified liposomes to surface-immobilized probe-DNA. With the lipid composition of each liposome encoding a unique target-DNA sequence, TOF-SIMS analysis was used to determine the chemical fingerprint of the bound liposomes. Using high-resolution TOF-SIMS imaging, providing sub-200 nm spatial resolution, single DNA targets could be detected and identified via the chemical fingerprint of individual liposomes. The results also demonstrate the capability of TOF-SIMS to provide multiplexed detection of DNA targets on substrate areas in the micrometer range. Together with a high multiplexing capacity, this makes the concept an interesting alternative to existing barcode concepts based on fluorescence, Raman, or graphical codes for small-scale bioanalysis.

nanoparticle probes

liposome

barcode

high-density

maldi-tof

expression

TOF-SIMS

targets

DNA

sims

arrays

hybridization

single molecule detection

Author

Anders Gunnarsson

Chalmers, Applied Physics, Biological Physics

Peter Sjövall

Chalmers, Applied Physics, Biological Physics

Fredrik Höök

Chalmers, Applied Physics, Biological Physics

Nano Letters

1530-6984 (ISSN) 1530-6992 (eISSN)

Vol. 10 2 732-737

Subject Categories

Physical Sciences

DOI

10.1021/nl904208y

More information

Created

10/6/2017