Liposome-Based Chemical Barcodes for Single Molecule DNA Detection Using Imaging Mass Spectrometry
Artikel i vetenskaplig tidskrift, 2010

We report on a mass-spectrometry (time-of-flight secondary ion mass spectrometry, TOF-SIMS) based method For multiplexed DNA detection utilizing a random array, where the lipid composition of small unilamellar liposomes act as chemical barcodes to identify unique DNA target sequences down to the single molecule level. In a sandwich format, suspended target-DNA to be detected mediates the binding of capture-DNA modified liposomes to surface-immobilized probe-DNA. With the lipid composition of each liposome encoding a unique target-DNA sequence, TOF-SIMS analysis was used to determine the chemical fingerprint of the bound liposomes. Using high-resolution TOF-SIMS imaging, providing sub-200 nm spatial resolution, single DNA targets could be detected and identified via the chemical fingerprint of individual liposomes. The results also demonstrate the capability of TOF-SIMS to provide multiplexed detection of DNA targets on substrate areas in the micrometer range. Together with a high multiplexing capacity, this makes the concept an interesting alternative to existing barcode concepts based on fluorescence, Raman, or graphical codes for small-scale bioanalysis.

nanoparticle probes

liposome

barcode

high-density

maldi-tof

expression

TOF-SIMS

targets

DNA

sims

arrays

hybridization

single molecule detection

Författare

Anders Gunnarsson

Chalmers, Teknisk fysik, Biologisk fysik

Peter Sjövall

Chalmers, Teknisk fysik, Biologisk fysik

Fredrik Höök

Chalmers, Teknisk fysik, Biologisk fysik

Nano Letters

1530-6984 (ISSN) 1530-6992 (eISSN)

Vol. 10 732-737

Ämneskategorier

Fysik

DOI

10.1021/nl904208y