Time-Resolved Thickness and Shape-Change Quantification using a Dual-Band Nanoplasmonic Ruler with Sub-Nanometer Resolution
Journal article, 2022
Time-resolved measurements of changes in the size and shape of nanobiological objects and layers are crucial to understand their properties and optimize their performance. Optical sensing is particularly attractive with high throughput and sensitivity, and label-free operation. However, most state-of-the-art solutions require intricate modeling or multiparameter measurements to disentangle conformational or thickness changes of biomolecular layers from complex interfacial refractive index variations. Here, we present a dual-band nanoplasmonic ruler comprising mixed arrays of plasmonic nanoparticles with spectrally separated resonance peaks. As electrodynamic simulations and model experiments show, the ruler enables real-time simultaneous measurements of thickness and refractive index variations in uniform and heterogeneous layers with sub-nanometer resolution. Additionally, nanostructure shape changes can be tracked, as demonstrated by quantifying the degree of lipid vesicle deformation at the critical coverage prior to rupture and supported lipid bilayer formation. In a broader context, the presented nanofabrication approach constitutes a generic route for multimodal nanoplasmonic optical sensing.
supported lipid bilayer
nanorulers
conformation
biosensors
nanoplasmonic sensors
biomolecules
Author
Ferry Nugroho
Vrije Universiteit Amsterdam
Chalmers, Physics, Chemical Physics
Universitas Indonesia
D. Switlik
University of Warsaw
Antonius Armanious
Swiss Federal Institute of Technology in Zürich (ETH)
Padraic O'Reilly
Chalmers, Physics, Chemical Physics
Iwan Darmadi
Chalmers, Physics, Chemical Physics
Sara Nilsson
Chalmers, Physics, Chemical Physics
Vladimir Zhdanov
Chalmers, Physics
Russian Academy of Sciences
Fredrik Höök
Chalmers, Physics, Nano and Biophysics
Tomasz Antosiewicz
University of Warsaw
Chalmers, Physics, Bionanophotonics
Christoph Langhammer
Chalmers, Physics, Chemical Physics
ACS Nano
1936-0851 (ISSN) 1936-086X (eISSN)
Vol. In PressRambidrag inom utlysningen "Materials Science 2015"
Swedish Foundation for Strategic Research (SSF) (RMA15-0052), 2016-05-01 -- 2021-06-30.
Subject Categories
Physical Chemistry
Atom and Molecular Physics and Optics
Other Physics Topics
DOI
10.1021/acsnano.2c04948
PubMed
36083800