Time-Resolved Thickness and Shape-Change Quantification using a Dual-Band Nanoplasmonic Ruler with Sub-Nanometer Resolution
Journal article, 2022

Time-resolved measurements of changes in the size and shape of nanobiological objects and layers are crucial to understand their properties and optimize their performance. Optical sensing is particularly attractive with high throughput and sensitivity, and label-free operation. However, most state-of-the-art solutions require intricate modeling or multiparameter measurements to disentangle conformational or thickness changes of biomolecular layers from complex interfacial refractive index variations. Here, we present a dual-band nanoplasmonic ruler comprising mixed arrays of plasmonic nanoparticles with spectrally separated resonance peaks. As electrodynamic simulations and model experiments show, the ruler enables real-time simultaneous measurements of thickness and refractive index variations in uniform and heterogeneous layers with sub-nanometer resolution. Additionally, nanostructure shape changes can be tracked, as demonstrated by quantifying the degree of lipid vesicle deformation at the critical coverage prior to rupture and supported lipid bilayer formation. In a broader context, the presented nanofabrication approach constitutes a generic route for multimodal nanoplasmonic optical sensing.

supported lipid bilayer

nanorulers

conformation

biosensors

nanoplasmonic sensors

biomolecules

Author

Ferry Nugroho

Vrije Universiteit Amsterdam

Chalmers, Physics, Chemical Physics

Universitas Indonesia

D. Switlik

University of Warsaw

Antonius Armanious

Swiss Federal Institute of Technology in Zürich (ETH)

Padraic O'Reilly

Chalmers, Physics, Chemical Physics

Iwan Darmadi

Chalmers, Physics, Chemical Physics

Sara Nilsson

Chalmers, Physics, Chemical Physics

Vladimir Zhdanov

Chalmers, Physics

Russian Academy of Sciences

Fredrik Höök

Chalmers, Physics, Nano and Biophysics

Tomasz Antosiewicz

University of Warsaw

Chalmers, Physics, Bionanophotonics

Christoph Langhammer

Chalmers, Physics, Chemical Physics

ACS Nano

1936-0851 (ISSN) 1936-086X (eISSN)

Vol. In Press

Rambidrag inom utlysningen "Materials Science 2015"

Swedish Foundation for Strategic Research (SSF) (RMA15-0052), 2016-05-01 -- 2021-06-30.

Subject Categories

Physical Chemistry

Atom and Molecular Physics and Optics

Other Physics Topics

DOI

10.1021/acsnano.2c04948

PubMed

36083800

More information

Latest update

10/3/2022