Melting transition of oriented Li-DNA fibers submerged in ethanol solutions
Journal article, 2021
The melting transition of Li-DNA fibers immersed in ethanol-water solutions has been studied using calorimetry and neutron diffraction techniques. The data have been analyzed using the Peyrard-Bishop-Dauxois model to determine the strengths of the intra- and inter-base pair potentials. The data and analysis show that the potentials are weaker than those for DNA in water. They become weaker still and the DNA less stable as the ethanol concentration increases but, conversely, the fibers become more compact and the distances between base pairs become more regular. The results show that the melting transition is relatively insensitive to local confinement and depends more on the interaction between the DNA and its aqueous environment.
thermodynamic modeling
DNA melting
calorimetry
neuron scattering
Author
Adrian Gonzalez Rodriguez
Chalmers, Physics, Nano and Biophysics
Institut Laue-Langevin
Universidad de Burgos
Andrew R. Wildes
Institut Laue-Langevin
Estelle Mossou
Institut Laue-Langevin
Viviana Cristiglio
Institut Laue-Langevin
Gaël Moiroux
Grenoble Alpes University
Centre national de la recherche scientifique (CNRS)
Jean Luc Garden
Grenoble Alpes University
Centre national de la recherche scientifique (CNRS)
Santiago Cuesta-López
Universidad de Burgos
ICAMCyL Foundation
Nikos Theodorakopoulos
University of Konstanz
Theoretical and Physical Chemistry
Michel Peyrard
École Normale Supérieure de Lyon
Biopolymers
0006-3525 (ISSN) 1097-0282 (eISSN)
Vol. 112 3 e23422Subject Categories
Physical Chemistry
Biophysics
Oceanography, Hydrology, Water Resources
DOI
10.1002/bip.23422