Melting transition of oriented Li-DNA fibers submerged in ethanol solutions
Artikel i vetenskaplig tidskrift, 2021
The melting transition of Li-DNA fibers immersed in ethanol-water solutions has been studied using calorimetry and neutron diffraction techniques. The data have been analyzed using the Peyrard-Bishop-Dauxois model to determine the strengths of the intra- and inter-base pair potentials. The data and analysis show that the potentials are weaker than those for DNA in water. They become weaker still and the DNA less stable as the ethanol concentration increases but, conversely, the fibers become more compact and the distances between base pairs become more regular. The results show that the melting transition is relatively insensitive to local confinement and depends more on the interaction between the DNA and its aqueous environment.
calorimetry
DNA melting
thermodynamic modeling
neuron scattering
Författare
Adrian Gonzalez Rodriguez
Universidad de Burgos
Institut Laue-Langevin
Chalmers, Fysik, Nano- och biofysik
Andrew R. Wildes
Institut Laue-Langevin
Estelle Mossou
Institut Laue-Langevin
Viviana Cristiglio
Institut Laue-Langevin
Gaël Moiroux
Centre national de la recherche scientifique (CNRS)
Université Grenoble Alpes
Jean Luc Garden
Centre national de la recherche scientifique (CNRS)
Université Grenoble Alpes
Santiago Cuesta-López
Universidad de Burgos
ICAMCyL Foundation
Nikos Theodorakopoulos
Theoretical and Physical Chemistry
Universität Konstanz
Michel Peyrard
Ecole Normale Superieure de Lyon
Biopolymers
0006-3525 (ISSN) 1097-0282 (eISSN)
Vol. In PressÄmneskategorier
Fysikalisk kemi
Biofysik
Oceanografi, hydrologi, vattenresurser
DOI
10.1002/bip.23422