A tandem giant magnetoresistance assay for one-shot quantification of clinically relevant concentrations of N-terminal pro-B-type natriuretic peptide in human blood
Journal article, 2021
We report a microfluidic sandwich immunoassay constructed around a dual-giant magnetoresistance (GMR) sensor array to quantify the heart failure biomarker NT-proBNP in human plasma at the clinically relevant concentration levels between 15 pg/mL and 40 ng/mL. The broad dynamic range was achieved by differential coating of two identical GMR sensors operated in tandem, and combining two standard curves. The detection limit was determined as 5 pg/mL. The assay, involving 53 plasma samples from patients with different cardiovascular diseases, was validated against the Roche Cobas e411 analyzer. The salient features of this system are its wide concentration range, low detection limit, small sample volume requirement (50 μL), and the need for a short measurement time of 15 min, making it a prospective candidate for practical use in point of care analysis.
Microfluidic
GMR
Biosensor
POCT
NT-proBNP
Author
Fanda Meng
Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry
Shandong First Medical University
Shandong Qianfoshan Hospital
Weisong Huo
Dongguan Bosh Biotechnologies
Jie Lian
People's Public Security University of China
Lei Zhang
Dongguan Bosh Biotechnologies
Xizeng Shi
Dongguan Bosh Biotechnologies
Aldo Jesorka
Chalmers, Chemistry and Chemical Engineering
Yunhua Gao
Chinese Academy of Sciences
Analytical and Bioanalytical Chemistry
1618-2642 (ISSN) 1618-2650 (eISSN)
Vol. 413 11 2943-2949Subject Categories
Medical Laboratory and Measurements Technologies
Analytical Chemistry
Biomedical Laboratory Science/Technology
DOI
10.1007/s00216-021-03227-5
PubMed
33624128