Synthesis of a precursor of D-fagomine by immobilized fructose-6-phosphate aldolase
Journal article, 2021

Fructose-6-phosphate aldolase (FSA) is an important enzyme for the C-C bond-forming reactions in organic synthesis. The present work is focused on the synthesis of a precursor of D-fagomine catalyzed by a mutant FSA. The biocatalyst has been immobilized onto several supports: magnetic nanoparticle clusters (mNC), cobalt-chelated agarose (Co-IDA), amino-functionalized agarose (MANA-agarose) and glyoxal-agarose, obtaining a 29.0%, 93.8%, 89.7% and 53.9% of retained activity, respectively. Glyoxal-agarose FSA derivative stood up as the best option for the synthesis of the precursor of D-fagomine due to the high reaction rate, conversion, yield and operational stability achieved. FSA immobilized in glyoxal-agarose could be reused up to 6 reaction cycles reaching a 4-fold improvement in biocatalyst yield compared to the non-immobilized enzyme.

Author

Gerard Masdeu Gámez

Chalmers, Chemistry and Chemical Engineering, Applied Chemistry

Universitat Autonoma de Barcelona (UAB)

Luis Miguel Vázquez

Universitat Autonoma de Barcelona (UAB)

Josep López-Santín

Universitat Autonoma de Barcelona (UAB)

Gloria Caminal

CSIC - Instituto de Quimica Avanzada de Cataluna (IQAC)

Slavko Kralj

Jozef Stefan Institute

Darko Makovec

Jozef Stefan Institute

Gregorio Álvaro

Universitat Autonoma de Barcelona (UAB)

Marina Guillén

Universitat Autonoma de Barcelona (UAB)

PLoS ONE

1932-6203 (ISSN) 19326203 (eISSN)

Vol. 16 4 April 2021 e0250513

Subject Categories

Other Basic Medicine

Biocatalysis and Enzyme Technology

Organic Chemistry

DOI

10.1371/journal.pone.0250513

PubMed

33886681

More information

Latest update

5/6/2021 2