Synthesis of a precursor of D-fagomine by immobilized fructose-6-phosphate aldolase
Artikel i vetenskaplig tidskrift, 2021

Fructose-6-phosphate aldolase (FSA) is an important enzyme for the C-C bond-forming reactions in organic synthesis. The present work is focused on the synthesis of a precursor of D-fagomine catalyzed by a mutant FSA. The biocatalyst has been immobilized onto several supports: magnetic nanoparticle clusters (mNC), cobalt-chelated agarose (Co-IDA), amino-functionalized agarose (MANA-agarose) and glyoxal-agarose, obtaining a 29.0%, 93.8%, 89.7% and 53.9% of retained activity, respectively. Glyoxal-agarose FSA derivative stood up as the best option for the synthesis of the precursor of D-fagomine due to the high reaction rate, conversion, yield and operational stability achieved. FSA immobilized in glyoxal-agarose could be reused up to 6 reaction cycles reaching a 4-fold improvement in biocatalyst yield compared to the non-immobilized enzyme.

Författare

Gerard Masdeu Gámez

Chalmers, Kemi och kemiteknik, Tillämpad kemi, Farmaceutisk teknologi

Universitat Autonoma de Barcelona (UAB)

Luis Miguel Vázquez

Universitat Autonoma de Barcelona (UAB)

Josep López-Santín

Universitat Autonoma de Barcelona (UAB)

Gloria Caminal

CSIC - Instituto de Quimica Avanzada de Cataluna (IQAC)

Slavko Kralj

Institut Jožef Stefan

Darko Makovec

Institut Jožef Stefan

Gregorio Álvaro

Universitat Autonoma de Barcelona (UAB)

Marina Guillén

Universitat Autonoma de Barcelona (UAB)

PLoS ONE

1932-6203 (ISSN)

Vol. 16 4 April 2021 e0250513

Ämneskategorier

Annan medicinsk grundvetenskap

Biokatalys och enzymteknik

Organisk kemi

DOI

10.1371/journal.pone.0250513

PubMed

33886681

Mer information

Senast uppdaterat

2021-05-06