Cultivation of edible filamentous fungus Aspergillus oryzae on volatile fatty acids derived from anaerobic digestion of food waste and cow manure
Journal article, 2021

In a circular economy approach, edible filamentous fungi (single cell protein) can be cultivated on volatile fatty acids (VFAs) derived from anaerobic digestion (AD) of organic-rich waste streams. In this study, the effect of pH, concentration/distribution of VFAs, nutrient supplementation, and type of waste on Aspergillus oryzae cultivation on synthetic VFAs, and actual VFAs derived from AD of food waste and cow manure were investigated. The optimal pH for A. oryzae growth on VFAs were 6 and 7 with maximum acetic acid consumption rates of 0.09 g/L.h. The fungus could thrive on high concentrations of acetic (up to 9 g/L) yielding 0.29 g dry biomass/gVFAsfed. In mixed VFAs cultures, A. oryzae primarily consumed caproic and acetic acids reaching a biomass yield of 0.26 g dry biomass/gVFAsfed (containing up to 41% protein). For waste-derived VFAs at pH 6, the fungus successfully consumed 81–100% of caproic, acetic, and butyric acids.

Aspergillus oryzae

Anaerobic digestion

Food waste

Edible filamentous fungi

Volatile fatty acids

Author

Clarisse Uwineza

University of Borås

Amir Mahboubi

University of Borås

Amelia Atmowidjojo

Faculty of Agriculture Technology

Alya Ramadhani

Faculty of Agriculture Technology

Steven Wainaina

University of Borås

Ria Millati

Faculty of Agriculture Technology

Rahma wikandari

Faculty of Agriculture Technology

Claes Niklasson

Chalmers, Chemistry and Chemical Engineering, Chemical Technology, Chemical Process and Reaction Engineering

Mohammad Taherzadeh Esfahani

University of Borås

Bioresource Technology

0960-8524 (ISSN)

Vol. 337 125410

Subject Categories

Food Science

Organic Chemistry

Other Industrial Biotechnology

DOI

10.1016/j.biortech.2021.125410

PubMed

34157433

More information

Latest update

7/15/2021