Quantification of single-strand DNA lesions caused by the topoisomerase II poison etoposide using single DNA molecule imaging
Journal article, 2022

DNA-damaging agents, such as radiation and chemotherapy, are common in cancer treatment, but the dosing has proven to be challenging, leading to severe side effects in some patients. Hence, to be able to personalize DNA-damaging chemotherapy, it is important to develop fast and reliable methods to measure the resulting DNA damage in patient cells. Here, we demonstrate how single DNA molecule imaging using fluorescence microscopy can quantify DNA-damage caused by the topoisomerase II (TopoII) poison etoposide. The assay uses an enzyme cocktail consisting of base excision repair (BER) enzymes to repair the DNA damage caused by etoposide and label the sites using a DNA polymerase and fluorescently labeled nucleotides. Using this DNA-damage detection assay we find a large variation in etoposide induced DNA-damage after in vitro treatment of blood cells from healthy individuals. We furthermore used the TopoII inhibitor ICRF-193 to show that the etoposide-induced damage in DNA was TopoII dependent. We discuss how our results support a potential future use of the assay for personalized dosing of chemotherapy.

Single-strand breaks

DNA damage

Base excision repair

Single molecule imaging

Chemotherapy

Nick translation

Author

Vandana Singh

Sahlgrenska University Hospital

Chalmers, Biology and Biological Engineering, Chemical Biology

Pegah Johansson

Sahlgrenska University Hospital

University of Gothenburg

Elina Ekedahl

Yii Lih Lin

Chalmers, Biology and Biological Engineering, Chemical Biology

Ola Hammarsten

Sahlgrenska University Hospital

University of Gothenburg

Fredrik Westerlund

Chalmers, Biology and Biological Engineering, Chemical Biology

Biochemical and Biophysical Research Communications

0006-291X (ISSN) 1090-2104 (eISSN)

Vol. 594 57-62

Subject Categories

Other Basic Medicine

Pharmacology and Toxicology

Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

DOI

10.1016/j.bbrc.2022.01.041

PubMed

35074586

More information

Latest update

2/1/2022 9