An oomycete NLP cytolysin forms transient small pores in lipid membranes
Journal article, 2022

Microbial plant pathogens secrete a range of effector proteins that damage host plants and consequently constrain global food production. Necrosis and ethylene-inducing peptide 1-like proteins (NLPs) are produced by numerous phytopathogenic microbes that cause important crop diseases. Many NLPs are cytolytic, causing cell death and tissue necrosis by disrupting the plant plasma membrane. Here, we reveal the unique molecular mechanism underlying the membrane damage induced by the cytotoxic model NLP. This membrane disruption is a multistep process that includes electrostatic-driven, plant-specific lipid recognition, shallow membrane binding, protein aggregation, and transient pore formation. The NLP-induced damage is not caused by membrane reorganization or large-scale defects but by small membrane ruptures. This distinct mechanism of lipid membrane disruption is highly adapted to effectively damage plant cells.

Author

Katja Pirc

National Institute of Chemistry

Luke A. Clifton

STFC Rutherford Appleton Laboratory

Neval Yilmaz

RIKEN

Andrea Saltalamacchia

Scuola Internazionale Superiore di Studi Avanzati

Mojca Mally

University of Ljubljana

Tina Snoj

National Institute of Chemistry

Nada Žnidaršič

University of Ljubljana

Marija Srnko

National Institute of Chemistry

Jure Borišek

National Institute of Chemistry

Petteri Parkkila

University of Helsinki

Chalmers, Physics, Nano and Biophysics

Isabell Albert

University of Erlangen-Nuremberg (FAU)

University of Tübingen

Marjetka Podobnik

National Institute of Chemistry

Keiji Numata

RIKEN

Thorsten Nürnberger

University of Johannesburg

University of Tübingen

Tapani Viitala

University of Helsinki

Jure Derganc

University of Ljubljana

Alessandra Magistrato

Scuola Internazionale Superiore di Studi Avanzati

Laboratorio Nazionale TASC

Jeremy H. Lakey

Newcastle University

Gregor Anderluh

National Institute of Chemistry

Science advances

2375-2548 (eISSN)

Vol. 8 10 eabj9406- eabj9406

Subject Categories

Cell Biology

Biochemistry and Molecular Biology

Plant Biotechnology

DOI

10.1126/sciadv.abj9406

PubMed

35275729

More information

Latest update

4/5/2022 7