An oomycete NLP cytolysin forms transient small pores in lipid membranes
Artikel i vetenskaplig tidskrift, 2022
Microbial plant pathogens secrete a range of effector proteins that damage host plants and consequently constrain global food production. Necrosis and ethylene-inducing peptide 1-like proteins (NLPs) are produced by numerous phytopathogenic microbes that cause important crop diseases. Many NLPs are cytolytic, causing cell death and tissue necrosis by disrupting the plant plasma membrane. Here, we reveal the unique molecular mechanism underlying the membrane damage induced by the cytotoxic model NLP. This membrane disruption is a multistep process that includes electrostatic-driven, plant-specific lipid recognition, shallow membrane binding, protein aggregation, and transient pore formation. The NLP-induced damage is not caused by membrane reorganization or large-scale defects but by small membrane ruptures. This distinct mechanism of lipid membrane disruption is highly adapted to effectively damage plant cells.
Författare
Katja Pirc
Kemijski Inštitut
Luke A. Clifton
STFC Rutherford Appleton Laboratory
Neval Yilmaz
RIKEN
Andrea Saltalamacchia
Scuola Internazionale Superiore di Studi Avanzati
Mojca Mally
Univerza V Ljubljani
Tina Snoj
Kemijski Inštitut
Nada Žnidaršič
Univerza V Ljubljani
Marija Srnko
Kemijski Inštitut
Jure Borišek
Kemijski Inštitut
Petteri Parkkila
Helsingin Yliopisto
Chalmers, Fysik, Nano- och biofysik
Isabell Albert
Friedrich-Alexander-Universität Erlangen Nurnberg (FAU)
Universität Tübingen
Marjetka Podobnik
Kemijski Inštitut
Keiji Numata
RIKEN
Thorsten Nürnberger
University of Johannesburg
Universität Tübingen
Tapani Viitala
Helsingin Yliopisto
Jure Derganc
Univerza V Ljubljani
Alessandra Magistrato
Scuola Internazionale Superiore di Studi Avanzati
Laboratorio Nazionale TASC
Jeremy H. Lakey
Newcastle University
Gregor Anderluh
Kemijski Inštitut
Science advances
2375-2548 (eISSN)
Vol. 8 10 eabj9406- eabj9406Ämneskategorier
Cellbiologi
Biokemi och molekylärbiologi
Växtbioteknologi
DOI
10.1126/sciadv.abj9406
PubMed
35275729