Dynamics of the normal gut microbiota: A longitudinal one-year population study in Sweden
Journal article, 2022
Temporal dynamics of the gut microbiota potentially limit the identification of microbial features associated with health status. Here, we used whole-genome metagenomic and 16S rRNA gene sequencing to characterize the intra- and inter-individual variations of gut microbiota composition and functional potential of a disease-free Swedish population (n = 75) over one year. We found that 23% of the total compositional variance was explained by intra-individual variation. The degree of intra-individual compositional variability was negatively associated with the abundance of Faecalibacterium prausnitzii (a butyrate producer) and two Bifidobacterium species. By contrast, the abundance of facultative anaerobes and aerotolerant bacteria such as Escherichia coli and Lactobacillus acidophilus varied extensively, independent of compositional stability. The contribution of intra-individual variance to the total variance was greater for functional pathways than for microbial species. Thus, reliable quantification of microbial features requires repeated samples to address the issue of intra-individual variations of the gut microbiota.
gut microbiota
biomarker
healthy microbiota
stability
Proteobacteria
Enterobacteriaceae
Author
Lisa M. Olsson
Wallenberg Lab.
Fredrik Boulund
Karolinska Institutet
Staffan Nilsson
University of Gothenburg
Chalmers, Mathematical Sciences, Applied Mathematics and Statistics
Muhammad Tanweer Khan
Wallenberg Lab.
Anders Gummesson
Wallenberg Lab.
Sahlgrenska University Hospital
L. Fagerberg
Royal Institute of Technology (KTH)
Lars Engstrand
Science for Life Laboratory (SciLifeLab)
Karolinska Institutet
Rosie Perkins
Wallenberg Lab.
Mathias Uhlen
Technical University of Denmark (DTU)
Royal Institute of Technology (KTH)
Göran Bergström
Sahlgrenska University Hospital
Wallenberg Lab.
Valentina Tremaroli
Wallenberg Lab.
Fredrik Bäckhed
Wallenberg Lab.
Sahlgrenska University Hospital
University of Copenhagen
Cell Host and Microbe
1931-3128 (ISSN) 19346069 (eISSN)
Vol. 30 5 726-739.e3Subject Categories
Microbiology
DOI
10.1016/j.chom.2022.03.002
PubMed
35349787