Assembly of Aggregation-Induced Emission Active Bola-Amphiphilic Macromolecules into Luminescent Nanoparticles Optimized for Two-Photon Microscopy In Vivo
Journal article, 2022
The (Z) and (E)-isomers of an extended tetraphenylethylene-based chromophore with optimized two-photon-induced luminescence properties are separated and functionalized with water-solubilizing pendant polymer groups, promoting their self-assembly in physiological media in the form of small, colloidal stable organic nanoparticles. The two resulting fluorescent suspensions are then evaluated as potential two-photon luminescent contrast agents for intravital epifluorescence and two-photon fluorescence microscopy. Comparisons with previously reported works involving similar fluorophores devoid of polymer side chains illustrate the benefits of later functionalization regarding the control of the self-assembly of the nano-objects and ultimately their biocompatibility toward the imaged organism.
Author
Jean Rouillon
Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry
Lamiaa M. A. Ali
University of Montpellier
Alexandria University
Kamel Hadj-Kaddour
University of Montpellier
Raphael Marie-Luce
Université de Lyon
Guillaume Simon
Université de Lyon
Melanie Onofre
University of Montpellier
Sandrine Denis Quanquin
Université de Lyon
Marion Jean
Aix Marseille University
Muriel Albalat
Aix Marseille University
Nicolas Vanthuyne
Aix Marseille University
Guillaume Micouin
Université de Lyon
Akos Banyasz
Université de Lyon
Magali Gary-Bobo
University of Montpellier
Cyrille Monnereau
Université de Lyon
Chantal Andraud
Université de Lyon
Biomacromolecules
1525-7797 (ISSN) 1526-4602 (eISSN)
Vol. 23 6 2485-2495Subject Categories
Physical Chemistry
Biochemistry and Molecular Biology
DOI
10.1021/acs.biomac.2c00232
PubMed
35608946