A p-Coumaroyl-CoA Biosensor for Dynamic Regulation of Naringenin Biosynthesis in Saccharomyces cerevisiae
Journal article, 2022

In vivo biosensors that can convert metabolite concentrations into measurable output signals are valuable tools for high-throughput screening and dynamic pathway control in the field of metabolic engineering. Here, we present a novel biosensor in Saccharomyces cerevisiae that is responsive to p-coumaroyl-CoA, a central precursor of many flavonoids. The sensor is based on the transcriptional repressor CouR from Rhodopseudomonas palustris and was applied in combination with a previously developed malonyl-CoA biosensor for dual regulation of p-coumaroyl-CoA synthesis within the naringenin production pathway. Using this approach, we obtained a naringenin titer of 47.3 mg/L upon external precursor feeding, representing a 15-fold increase over the nonregulated system.

transcription repressor

yeast

flavonoids

transcriptional regulation

dynamic pathway control

Author

Dany Liu

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Maria Sole Sica

Jiwei Mao

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Fang Chao

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Verena Siewers

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

ACS Synthetic Biology

2161-5063 (eISSN)

Vol. 11 10 3228-3238

Synthetic microbial consortia-based platform for flavonoids production using synthetic biology (Synbio4Flav)

European Commission (EC) (EC/H2020/814650), 2019-01-01 -- 2023-02-28.

Infrastructure

Chalmers Infrastructure for Mass spectrometry

Subject Categories

Other Engineering and Technologies not elsewhere specified

Control Engineering

Other Electrical Engineering, Electronic Engineering, Information Engineering

DOI

10.1021/acssynbio.2c00111

PubMed

36137537

More information

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3/7/2024 9