Stimuli-Responsive Membrane Anchor Peptide Nanofoils for Tunable Membrane Association and Lipid Bilayer Fusion
Journal article, 2022
Self-assembled peptide nanostructures with stimuli-responsive features are promising as functional materials. Despite extensive research efforts, water-soluble supramolecular constructs that can interact with lipid membranes in a controllable way are still challenging to achieve. Here, we have employed a short membrane anchor protein motif (GLFD) and coupled it to a spiropyran photoswitch. Under physiological conditions, these conjugates assemble into ∼3.5 nm thick, foil-like peptide bilayer morphologies. Photoisomerization from the closed spiro (SP) form to the open merocyanine (MC) form of the photoswitch triggers rearrangements within the foils. This results in substantial changes in their membrane-binding properties, which also varies sensitively to lipid composition, ranging from reversible nanofoil reformation to stepwise membrane adsorption. The formed peptide layers in the assembly are also able to attach to various liposomes with different surface charges, enabling the fusion of their lipid bilayers. Here, SP-to-MC conversion can be used both to trigger and to modulate the liposome fusion efficiency.
liposomes
spiropyran
lipid bilayer fusion
membrane activity
self-assembly
peptide bilayer
Author
Vignesh Udyavara Nagaraj
Eötvös Loránd University (ELTE)
Hungarian Academy of Sciences
Tünde Juhász
Hungarian Academy of Sciences
Mayra Quemé-Peña
Eötvös Loránd University (ELTE)
Hungarian Academy of Sciences
Imola Cs Szigyártó
Hungarian Academy of Sciences
Dóra Bogdán
Hungarian Academy of Sciences
Semmelweis University
András Wacha
Hungarian Academy of Sciences
Judith Mihály
Hungarian Academy of Sciences
Lorand Romanszki
Hungarian Academy of Sciences
Zoltán Varga
Hungarian Academy of Sciences
Joakim Andreasson
Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry
István Mándity
Hungarian Academy of Sciences
Semmelweis University
Tamas Beke-Somfai
Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry
Hungarian Academy of Sciences
Published in
ACS Applied Materials & Interfaces
1944-8244 (ISSN) 1944-8252 (eISSN)
Vol. 14 Issue 50 p. 55320-55331Categorizing
Subject Categories (SSIF 2011)
Physical Chemistry
Other Chemistry Topics
Biophysics
Identifiers
DOI
10.1021/acsami.2c11946
PubMed
36473125