Stimuli-Responsive Membrane Anchor Peptide Nanofoils for Tunable Membrane Association and Lipid Bilayer Fusion
Artikel i vetenskaplig tidskrift, 2022
Self-assembled peptide nanostructures with stimuli-responsive features are promising as functional materials. Despite extensive research efforts, water-soluble supramolecular constructs that can interact with lipid membranes in a controllable way are still challenging to achieve. Here, we have employed a short membrane anchor protein motif (GLFD) and coupled it to a spiropyran photoswitch. Under physiological conditions, these conjugates assemble into ∼3.5 nm thick, foil-like peptide bilayer morphologies. Photoisomerization from the closed spiro (SP) form to the open merocyanine (MC) form of the photoswitch triggers rearrangements within the foils. This results in substantial changes in their membrane-binding properties, which also varies sensitively to lipid composition, ranging from reversible nanofoil reformation to stepwise membrane adsorption. The formed peptide layers in the assembly are also able to attach to various liposomes with different surface charges, enabling the fusion of their lipid bilayers. Here, SP-to-MC conversion can be used both to trigger and to modulate the liposome fusion efficiency.
liposomes
spiropyran
lipid bilayer fusion
membrane activity
self-assembly
peptide bilayer
Författare
Vignesh Udyavara Nagaraj
Eötvös Loránd University (ELTE)
Magyar Tudomanyos Akademia
Tünde Juhász
Magyar Tudomanyos Akademia
Mayra Quemé-Peña
Eötvös Loránd University (ELTE)
Magyar Tudomanyos Akademia
Imola Cs Szigyártó
Magyar Tudomanyos Akademia
Dóra Bogdán
Magyar Tudomanyos Akademia
Semmelweis Egyetem
András Wacha
Magyar Tudomanyos Akademia
Judith Mihály
Magyar Tudomanyos Akademia
Lorand Romanszki
Magyar Tudomanyos Akademia
Zoltán Varga
Magyar Tudomanyos Akademia
Joakim Andreasson
Chalmers, Kemi och kemiteknik, Kemi och biokemi
István Mándity
Magyar Tudomanyos Akademia
Semmelweis Egyetem
Tamas Beke-Somfai
Chalmers, Kemi och kemiteknik, Kemi och biokemi
Magyar Tudomanyos Akademia
ACS Applied Materials & Interfaces
1944-8244 (ISSN) 1944-8252 (eISSN)
Vol. 14 50 55320-55331Ämneskategorier
Fysikalisk kemi
Annan kemi
Biofysik
DOI
10.1021/acsami.2c11946
PubMed
36473125