Fish Protein Hydrolysate from Sulfated Polysaccharides Extraction Residue of Tuna Processing By-Products with Bioactive and Functional Properties
Journal article, 2023

The ethanol-induced precipitation after enzymatic hydrolysis commonly used for sulfated polysaccharide extraction from marine resources wastes a large amount of proteins. Here, possible extraction of fish protein hydrolysates (FPH) from the ethanol residue of sulfated polysaccharide precipitation from head, bone, and skin of skipjack tuna is investigated. Antioxidant, antibacterial, angiotensin I-converting enzyme (ACE) inhibitory activities and functional properties of the recovered FPHs are also evaluated. A degree of hydrolysis of 40.93, 38.13, and 37.23 is achieved for FPH from head, bone, and skin, respectively. FPH from the head presents the highest antioxidant and ACE inhibitory activity as well as foam/emulsion capacity among all the FPHs. The FPHs are all able to inhibit three Gram-positive bacteria and three Gram-negative bacteria to varying degrees and have a water solubility >65%. Altogether, the results demonstrate great potential for recovery of bioactive/functional peptides from the residue of sulfated polysaccharide extraction process enabling efficient biorefining of aquatic resources.

functional properties

ACE-inhibitory activity

fish by-products


protein hydrolysates


Shahab Naghdi

Tarbiat Modares University

Masoud Rezaei

Tarbiat Modares University

Mehdi Tabarsa

Tarbiat Modares University

Mehdi Abdollahi

Chalmers, Life Sciences, Food and Nutrition Science

Global Challenges

20566646 (eISSN)

Vol. In Press

Blue Biorefining: a holistic solution for increasing resource efficiency in seafood industry (BlueBio)

VINNOVA (2021-03724), 2021-12-01 -- 2024-12-01.

Subject Categories

Biochemistry and Molecular Biology

Biocatalysis and Enzyme Technology

Other Industrial Biotechnology



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