Cellulose-Derived Carbon Dots for Inner Filter Effect-Based Selective Sensing of Ofloxacin Antibiotics
Journal article, 2023

A two-step viable synthetic approach for quick and energy-efficient acid-based charring is described here to fabricate self-passivated fluorescent water-soluble CD (wsCD) from sustainable microcrystalline cellulose (MCC) materials. The aqueous solution of wsCD exhibits blue emission under UV-light illumination and shows a fluorescence quantum yield of ∼6%. The wsCD are used here for the selective sensing of ofloxacin (OFLX) from among the four tested antibiotics of the fluoroquinolone class, namely, ciprofloxacin (CPLX), ofloxacin (OFLX), levofloxacin (LVLX), and moxifloxacin (MXLX) based on the simpler fluorescence quenching experiment with a detection limit of ∼0.025 ppm. The plausible mechanism for the selective sensing of OFLX has been proposed based on a detailed analysis of absorbance and time-resolved photoluminescence spectroscopy which indicates the involvement of the inner-filter effect (IFE). Antimicrobial studies of wsCD were conducted on two bacterial strains (Escherichia coli and Staphylococcus aureus) and two fungal strains (Aspergillus niger and Penicillium chrysogenum), where wsCD did not show any toxic effects up to the concentration of 1 mg/mL, hence supporting their biocompatible behavior. Further, an antibiosis study involving the combination of the antibiotic and antifungal agents with wsCD against the growth of the same bacterial and fungal strains was conducted, where wsCD showed mild antifungal activity.

water-soluble carbon dots

antibiotics

fluorescence

cellulose

carbon dots

sensing

Author

Ruchi Aggarwal

Malaviya National Institute of Technology Jaipur

Anjali Kumari Garg

Malaviya National Institute of Technology Jaipur

Vishrant Kumar

Indian Institute of Science Education and Research Bhopal

Harshita Jonwal

Dr. B. Lal Institute of Biotechnology

Sonia Sethi

Dr. B. Lal Institute of Biotechnology

Srushti Gadiyaram

National Institute of Technology Kurukshetra

Sumit Kumar Sonkar

Malaviya National Institute of Technology Jaipur

Amit Kumar Sonker

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry

Gunnar Westman

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry

ACS Applied Nano Materials

25740970 (eISSN)

Vol. 6 8 6518-6527

Subject Categories

Other Chemistry Topics

DOI

10.1021/acsanm.2c05526

More information

Latest update

8/2/2023 1