Restricting Promiscuity of Plant Flavonoid 3'-Hydroxylase and 4'-O-Methyltransferase Improves the Biosynthesis of (2S)-Hesperetin in E. coli
Journal article, 2023
Enzyme promiscuity
flavonoid 3'-hydroxylase
directed evolution
flavonoid
flavonoid 4'-O-methyltransferase
(2S)-hesperetin
Author
Juan Liu
Chalmers, Life Sciences, Systems and Synthetic Biology
Zhiqiang Xiao
Hunan Academy of Agricultural Sciences
Hunan University
Hunan Key Lab Fruits & Vegetables Storage Proc Qua
Siqi Zhang
Hunan Academy of Agricultural Sciences
Hunan University
Hunan Key Lab Fruits & Vegetables Storage Proc Qua
Zhen Wang
Hunan University
Hunan Academy of Agricultural Sciences
Hunan Key Lab Fruits & Vegetables Storage Proc Qua
Yun Chen
Chalmers, Life Sciences, Systems and Synthetic Biology
Yang Shan
Hunan University
Hunan Academy of Agricultural Sciences
Hunan Key Lab Fruits & Vegetables Storage Proc Qua
Journal of Agricultural and Food Chemistry
0021-8561 (ISSN) 1520-5118 (eISSN)
Vol. 71 25 9826-9835Subject Categories
Biochemistry and Molecular Biology
Biocatalysis and Enzyme Technology
DOI
10.1021/acs.jafc.3c02071
PubMed
37310069