Polymer brush functionalised nanopore sensors
Licentiate thesis, 2024
This thesis works towards the goal of developing a platform for long-term, non-intrusive single-molecule measurements on proteins. This is done by the integration of ionic current nanopore sensing with functional nanostructures. A novel nanochamber was fabricated through a combination of electron beam lithography, wet etching and controlled breakdown. This nanochamber, consisting of a cavity connected to two nanopores, is designed for protein trapping, where the nanopores would act as “gates”.
Thus, the concept of macromolecular gating becomes central in protein trapping. The thesis demonstrates thermo-responsive gating for proteins using PNIPAM functionalised nanopore arrays, as well as voltage-gating for both DNA and proteins with a PEG-functionalised single nanopore sensor. The utilisation of a voltage-gated nanopore allows for ion current readout, confirming molecular translocation through the pore. To facilitate nanopore sensing at higher voltages beyond the gating threshold, a Fourier transform-based algorithm for ion current data filtering has been developed.
polymer brushes
nanopore sensing
nanofabrication
Author
Julia Järlebark
Chalmers, Chemistry and Chemical Engineering, Applied Chemistry
Stable trapping of multiple proteins at physiological conditions using nanoscale chambers with macromolecular gates
Nature Communications,;Vol. 14(2023)
Journal article
Polymer Brushes on Silica Nanostructures Prepared by Aminopropylsilatrane Click Chemistry: Superior Antifouling and Biofunctionality
ACS Applied Materials & Interfaces,;Vol. 15(2023)p. 10228-10239
Journal article
Single Molecule Analysis in Nanoscale ReactionChambers SIMONANO2
European Commission (EC) (EC/H2020/101001854), 2021-02-01 -- 2026-01-31.
Areas of Advance
Nanoscience and Nanotechnology
Subject Categories
Biochemistry and Molecular Biology
Biophysics
Nano Technology
Infrastructure
Nanofabrication Laboratory
Publisher
Chalmers
Carinas Pater Noster