Investigating formate tolerance mechanisms in Saccharomyces cerevisiae and its application
Journal article, 2023
Current global energy and environmental crisis have spurred efforts towards developing sustainable biotechnological solutions, such as utilizing CO2 and its derivatives as raw materials. Formate is an attractive one-carbon source due to its high solubility and low reduction potential. However, the regulatory mechanism of formate metabolism in yeast remains largely unexplored. This study employed adaptive laboratory evolution (ALE) to improve formate tolerance in Saccharomyces cerevisiae and characterized the underlying molecular mechanisms. The evolved strain was applied to produce free fatty acids (FFAs) under high concentration of formate with glucose addition. The results showed that the evolved strain achieved a FFAs titer of 250 mg/L. Overall, this study sheds light on the regulatory mechanism of formate tolerance and provides a platform for future studies under high concentrations of formate.
Formate tolerance
Adaptive laboratory evolution
Saccharomyces cerevisiae
Free fatty acids
Author
Kai Wang
Beijing University of Chemical Technology
Yining Liu
Beijing University of Chemical Technology
Zhuoheng Wu
Beijing University of Chemical Technology
Yilu Wu
Beijing University of Chemical Technology
Haoran Bi
Beijing University of Chemical Technology
Yanhui Liu
Beijing University of Chemical Technology
Meng Wang
Beijing University of Chemical Technology
Biqiang Chen
Beijing University of Chemical Technology
Jens B Nielsen
Chalmers, Life Sciences, Systems and Synthetic Biology
Beijing University of Chemical Technology
Zihe Liu
Beijing University of Chemical Technology
Tianwei Tan
Beijing University of Chemical Technology
Green Carbon
29501555 (eISSN)
Vol. 1 1 65-74Subject Categories (SSIF 2011)
Microbiology
DOI
10.1016/j.greenca.2023.08.003