Growth Rate Evaluation of the Budding Yeast Saccharomyces cerevisiae Cells Carrying Endogenously Expressed Fluorescent Protein Fusions
Book chapter, 2023
Fluorescent proteins within fluorescent fusions have been reported to affect cellular growth fitness via altering native protein function and intracellular localization. Here we report in detail a procedure to analyze the growth characteristics of yeast cells expressing such fusions in comparison to unmodified parental strain. This approach can serve as an initial step in fluorescent protein characterization in vivo.
Author
Kara L. Schneider
University of Gothenburg
Lucas E. Reibenspies
University of Regensburg
University of Gothenburg
Thomas Nystrom
University of Gothenburg
Sviatlana Shashkova
University of Gothenburg
Chalmers, Mathematical Sciences, Applied Mathematics and Statistics
Methods in Molecular Biology
10643745 (ISSN) 1940-6029 (eISSN)
Vol. 2564Subject Categories (SSIF 2025)
Molecular Biology
DOI
10.1007/978-1-0716-2667-2_10