Growth Rate Evaluation of the Budding Yeast Saccharomyces cerevisiae Cells Carrying Endogenously Expressed Fluorescent Protein Fusions
Kapitel i bok, 2023
Fluorescent proteins within fluorescent fusions have been reported to affect cellular growth fitness via altering native protein function and intracellular localization. Here we report in detail a procedure to analyze the growth characteristics of yeast cells expressing such fusions in comparison to unmodified parental strain. This approach can serve as an initial step in fluorescent protein characterization in vivo.
Författare
Kara L. Schneider
Göteborgs universitet
Lucas E. Reibenspies
Universität Regensburg
Göteborgs universitet
Thomas Nystrom
Göteborgs universitet
Sviatlana Shashkova
Göteborgs universitet
Chalmers, Matematiska vetenskaper, Tillämpad matematik och statistik
Methods in Molecular Biology
Vol. 2564
Ämneskategorier (SSIF 2025)
Molekylärbiologi
DOI
10.1007/978-1-0716-2667-2_10