Monitoring nucleoside metabolism in living cells with a nucleobase analogue via fluorescence lifetime imaging
Journal article, 2025
To overcome challenges in fluorescence labelling of RNA inside living cells we have recently introduced a direct approach using the fluorescent nucleobase analogue 2CNqA. Here we demonstrate its potential for use in fluorescence lifetime imaging (FLIM) to investigate nucleoside metabolism and for metabolic RNA labelling.
Author
Pauline Pfeiffer
Chemistry and Biochemistry Phd Students and Postdocs
Niusha Bagheri
AlbaNova University Center
Chen Qian
University of Gothenburg
Jerker Widengren
AlbaNova University Center
Marcus Wilhelmsson
Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry
Chemical Communications
1359-7345 (ISSN) 1364-548X (eISSN)
Vol. In PressEndogenous non-invasive fluorescence labeling of RNA and its use in cell imaging
Swedish Research Council (VR) (2021-04409), 2021-12-01 -- 2025-11-30.
Funktionell leverans av nukleotid-baserade läkemedel
Swedish Foundation for Strategic Research (SSF) (IRC15-0065), 2017-03-01 -- 2024-12-31.
Subject Categories (SSIF 2025)
Molecular Biology
Organic Chemistry
Physical Chemistry
Areas of Advance
Nanoscience and Nanotechnology
DOI
10.1039/d5cc03959b
PubMed
40888260
Related datasets
DOI: https://doi.org/10.1039/d5cc03959b