A gene expression fingerprint of mouse stomach ECL cells.
Journal article, 2005
Many of the endocrine cells in the stomach are poorly characterized with respect to physiological significance. In some cases, the anticipated hormone has not yet been identified. Global gene expression analysis of mouse stomach was performed in an attempt to identify the ECL-cell peptide/protein. Specific functional activation (omeprazole-induced hypergastrinaemia) was used as a tool to generate a gene expression fingerprint of the ECL cells. The proposed fingerprint includes 14 genes, among them six are known to be expressed by ECL cells (=positive controls), and some novel ones, which are likely to be ECL-cell-related. The known ECL-cell-related genes are those encoding histidine decarboxylase, chromogranin A and B, vesicular monoamine transporter 2, synaptophysin, and the cholecystokinin-B receptor. In addition, the fingerprint included five genes, which might be involved in the process of secretion and three ESTs with unknown function. Interestingly, parathyroid hormone-like hormone (Pthlh) was identified as a candidate ECL-cell peptide hormone.
Stomach
metabolism
metabolism
Female
Animals
chemically induced
Tissue Distribution
metabolism
metabolism
Omeprazole
Mice
Gastrins
Enteroendocrine Cells
Cultured
Proteome
Gastric Mucosa
metabolism
Stomach Diseases
Cells
Inbred C57BL
metabolism
methods
Peptide Mapping
metabolism
Peptide Hormones
Mice
Author
Niklas Andersson
University of Gothenburg
Sofia Movérare-Skrtic
University of Gothenburg
Rolf Håkanson
Claes Ohlsson
University of Gothenburg
Biochemical and Biophysical Research Communications
0006-291X (ISSN) 1090-2104 (eISSN)
Vol. 332 2 404-10Subject Categories
MEDICAL AND HEALTH SCIENCES
DOI
10.1016/j.bbrc.2005.05.004
PubMed
15896709