A gene expression fingerprint of mouse stomach ECL cells.
Artikel i vetenskaplig tidskrift, 2005

Many of the endocrine cells in the stomach are poorly characterized with respect to physiological significance. In some cases, the anticipated hormone has not yet been identified. Global gene expression analysis of mouse stomach was performed in an attempt to identify the ECL-cell peptide/protein. Specific functional activation (omeprazole-induced hypergastrinaemia) was used as a tool to generate a gene expression fingerprint of the ECL cells. The proposed fingerprint includes 14 genes, among them six are known to be expressed by ECL cells (=positive controls), and some novel ones, which are likely to be ECL-cell-related. The known ECL-cell-related genes are those encoding histidine decarboxylase, chromogranin A and B, vesicular monoamine transporter 2, synaptophysin, and the cholecystokinin-B receptor. In addition, the fingerprint included five genes, which might be involved in the process of secretion and three ESTs with unknown function. Interestingly, parathyroid hormone-like hormone (Pthlh) was identified as a candidate ECL-cell peptide hormone.

Stomach

metabolism

metabolism

Female

Animals

chemically induced

Tissue Distribution

metabolism

metabolism

Omeprazole

Mice

Gastrins

Enteroendocrine Cells

Cultured

Proteome

Gastric Mucosa

metabolism

Stomach Diseases

Cells

Inbred C57BL

metabolism

methods

Peptide Mapping

metabolism

Peptide Hormones

Mice

Författare

Niklas Andersson

Göteborgs universitet

Sofia Movérare-Skrtic

Göteborgs universitet

Rolf Håkanson

Claes Ohlsson

Göteborgs universitet

Biochemical and Biophysical Research Communications

0006-291X (ISSN) 1090-2104 (eISSN)

Vol. 332 2 404-10

Ämneskategorier

MEDICIN OCH HÄLSOVETENSKAP

DOI

10.1016/j.bbrc.2005.05.004

PubMed

15896709