Estrogen receptor specificity for the effects of estrogen in ovariectomized mice.
Journal article, 2002
Estrogen exerts a variety of important physiological effects, which have been suggested to be mediated via the two known estrogen receptors (ERs), alpha and beta. Three-month-old ovariectomized mice, lacking one or both of the two estrogen receptors, were given estrogen subcutaneously (2.3 micro g/mouse per day) and the effects on different estrogen-responsive parameters, including skeletal effects, were studied. We found that estrogen increased the cortical bone dimensions in both wild-type (WT) and double ER knockout (DERKO) mice. DNA microarray analysis was performed to characterize this effect on cortical bone and it identified four genes that were regulated by estrogen in both WT and DERKO mice. The effect of estrogen on cortical bone in DERKO mice might either be due to remaining ERalpha activity or represent an ERalpha/ERbeta-independent effect. Other effects of estrogen, such as increased trabecular bone mineral density, thymic atrophy, fat reduction and increased uterine weight, were mainly ERalpha mediated.
Mice
genetics
Bone Density
Thymus Gland
Receptors
alpha-Macroglobulins
Complement C3
Estrogen Receptor beta
Collagen Type VIII
Uterus
Mice
genetics
genetics
Sialoglycoproteins
Interleukin-3
analysis
drug effects
Organ Size
Matrix Metalloproteinase 9
drug effects
metabolism
genetics
Estrogen Receptor alpha
RNA
Female
Liver
genetics
cytology
genetics
Messenger
Bone and Bones
Knockout
Ovariectomy
Animals
pharmacology
drug effects
genetics
cytology
drug effects
physiology
Estrogen
Estrogens
Author
Marie K Lindberg
University of Gothenburg
Z Weihua
Niklas Andersson
University of Gothenburg
Sofia Movérare-Skrtic
University of Gothenburg
Hui-Ling Gao
O Vidal
M C Erlandsson
Sara H Windahl
University of Gothenburg
G Andersson
D B Lubahn
Catharina Lindholm
University of Gothenburg
Karin Dahlman-Wright
J-A Gustafsson
Claes Ohlsson
University of Gothenburg
Journal of Endocrinology
0022-0795 (ISSN) 14796805 (eISSN)
Vol. 174 2 167-78Subject Categories
MEDICAL AND HEALTH SCIENCES
PubMed
12176656