Comparison of PEI-PEG and PLL-PEG Copolymer Coatings on the Prevention of Protein Fouling
Journal article, 2009

The effect of surface charge on the protein resistance of adsorbed layers of poly(ethylene imine)-[g]poly(ethylene glycol), PEI-PEG, and poly(L-lysine)-[g]poly(ethylene glycol), PLL-PEG, was studied. Mixed and monofunctional self-assembled monolayers, SAMs, on gold were obtained by adsorption of 16-mercapto-1-hexadecanoic acid and 16-mercapto-1-hexadecanol. The surface charge was systematically varied by changing the ratio of the two alkanethiols. The graft copolymers PEI-PEG and PLL-PEG were adsorbed at the SAMs and tested for resistance towards human serum albumin and fibrinogen. The adsorbed amount of copolymers increased with increasing negative surface charge. However, the best protein resistance was found at an intermediate surface charge. The PLL-PEG covered Surfaces showed better protein resistance than the PEI-PEG covered surfaces. Thus, this work demonstrates that an adsorbed layer of PEG-grafted PEI and, in particular, PEG-grafted PLL is efficient in preventing protein adsorption when there is charge neutralization between the copolymer and the underlying surface.

poly(ethylene glycol)

self-assembled monolayers

poly(L-lysine)

poly(ethylene imine)

protein rejection

Author

Anna Bergstrand

Chalmers, Chemical and Biological Engineering, Applied Surface Chemistry

Ghazal Rahmani-Monfared

Chalmers

Åsa Östlund

Chalmers, Chemical and Biological Engineering, Applied Surface Chemistry

Magnus Nydén

Chalmers, Chemical and Biological Engineering, Applied Surface Chemistry

SuMo Biomaterials

Krister Holmberg

Chalmers, Chemical and Biological Engineering, Applied Surface Chemistry

Journal of Biomedical Materials Research - Part A

1549-3296 (ISSN)

Vol. 88 3 608-615

Subject Categories

Chemical Sciences

DOI

10.1002/jbm.a.31894

More information

Latest update

9/10/2018