Reconstruction of a bacterial isoprenoid biosynthetic pathway in Saccharomyces cerevisiae
Journal article, 2008

A eukaryotic mevalonate pathway transferred and expressed in Escherichia coli, and a mammalian hydrocortisone biosynthetic pathway rebuilt in Saccharomyces cerevisiae are examples showing that transferring metabolic pathways from one organism to another can have a powerful impact on cell properties. In this study, we reconstructed the E. coli isoprenoid biosynthetic pathway in S. cerevisiae. Genes encoding the seven enzymatic steps of the pathway were cloned and expressed in S. cerevisiae. mRNA from the seven genes was detected, and the pathway was shown able to sustain growth of yeast in conditions of inhibition of its constitutive isoprenoid biosynthetic pathway. © 2008 Federation of European Biochemical Societies.

2-Methyl erythritol 4-phosphate



Saccharomyces cerevisiae



J. Maury

Technical University of Denmark (DTU)

M. A. Asadollahi

Technical University of Denmark (DTU)

K. Moller

CMC Biopharmaceuticals A/S

M. Schalk

Firmenich Inc.

A. Clark

Firmenich Inc.

L. R. Formenti

Technical University of Denmark (DTU)

Jens B Nielsen

Chalmers, Chemical and Biological Engineering, Life Sciences

FEBS Letters

0014-5793 (ISSN) 18733468 (eISSN)

Vol. 582 29 4032-4038

Subject Categories

Biochemistry and Molecular Biology

Other Industrial Biotechnology

Areas of Advance

Life Science Engineering (2010-2018)



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9/6/2018 1