Towards an electrowetting-based digital microfluidic platform for magnetic immunoassays
Journal article, 2009
We demonstrate ElectroWetting-On-Dielectric (EWOD) transport and SQUID gradiometer detection of magnetic nanoparticles (MNPs) suspended in a 2 mu l de-ionized water droplet. This proof-of-concept methodology constitutes the first development step towards a highly sensitive magnetic immunoassay platform with SQUID readout and droplet-based sample handling. Magnetic AC-susceptibility measurements were performed on MNPs with a hydrodynamic diameter of 100 nm using a high-Tc dc Superconducting Quantum Interference Device (SQUID) gradiometer as detector. We observed that the signal amplitude per unit volume is 2.5 times higher for a 2 ml sample droplet compared to a 30 ml sample volume.
Author
Vincent Schaller
Chalmers, Applied Physics, Electronics Material and Systems
Anke Sanz-Velasco
Chalmers, Applied Physics, Electronics Material and Systems
Alexei Kalaboukhov
Chalmers, Microtechnology and Nanoscience (MC2), Quantum Device Physics
Justin Schneiderman
Chalmers, Microtechnology and Nanoscience (MC2), Quantum Device Physics
Fredrik Öisjöen
Chalmers, Microtechnology and Nanoscience (MC2), Quantum Device Physics
Aldo Jesorka
Chalmers, Chemical and Biological Engineering, Physical Chemistry
Andrea Prieto Astalan
Imego AB - The Institute of Micro and Nanotechnology
Anatol Krozer
Imego AB - The Institute of Micro and Nanotechnology
Cristina Rusu
Imego AB - The Institute of Micro and Nanotechnology
Peter Enoksson
Chalmers, Applied Physics, Electronics Material and Systems
Dag Winkler
Chalmers, Microtechnology and Nanoscience (MC2), Quantum Device Physics
Lab on a Chip - Miniaturisation for Chemistry and Biology
1473-0197 (ISSN) 1473-0189 (eISSN)
Vol. 9 23 3433-3436Areas of Advance
Nanoscience and Nanotechnology
Production
Life Science Engineering (2010-2018)
Subject Categories
Medical Laboratory and Measurements Technologies
Other Materials Engineering
Fluid Mechanics and Acoustics
Condensed Matter Physics
DOI
10.1039/b912646e