Robustness analysis of HOG pathway related genes in budding yeast
Conference poster, 2007

We applied a novel genetic screening method, named “genetic tug-of-war” (gTOW) to estimate the upper limit of gene copy numbers in Saccharomyces cerevisiae. The study involved 29 HOG pathway related genes which included kinases, phosphatases and transcription factors to cover different set of players in the signal transduction system. In addition a phenotypic profiling was conducted in four different growth conditions with three outputs: lag phase, growth phase and efficiency of growth. A number of interesting hits were identified, including PBS2 which had low numbers of gene copies. It will be of interest to expand the study to encompass the entire known signal transduction system in yeast to search for sensitive nodes.


Lars-Göran Ottosson

University of Gothenburg

Doryaneh Ahmadpour

University of Gothenburg

Marcus Krantz

University of Gothenburg

Jonas Warringer

University of Gothenburg

Anders Blomberg

University of Gothenburg

Hiroaki Kitano

Stefan Hohmann

University of Gothenburg

ICSB 2007 Conference Proceedings, 8th International Conference on Systems Biology (ICSB2007), October 1-6, 2007

Subject Categories

Cell Biology

Biochemistry and Molecular Biology

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