Robustness and fragility in the yeast High Osmolarity signal transduction pathway
Conference poster, 2008

Objective: The cellular signalling networks that integrate various environmental stimuli with information on cellular status must be robust to stimuli fluctuations as well as to stochastic differences in the amounts of signalling components. Here, we challenge the high osmolarity glycerol response (HOG) signal transduction pathway in the yeast Saccharomyces cerevisiae with systematic disturbances in components’ expression levels implemented by a “genetic tug-of-war”, or gTOW, methodology. Results: The disturbances were performed under various external perturbations, including pathway activation by osmotic shock. The resulting phenotypes in this particular study reflect a wide range of sensitivities, and disperse without any clear pattern over biochemical functions and pathway modules alike, with the most sensitive nodes being PBS2 and SSK1. Conclusions: Ideally, the obtained sensitivity profiles will allow us to impose parameter constraints. However, a more important aspect is the qualitative improvement of model structures, when local fragilities cannot be explained by the model structure. Surprisingly, the “neighboring” nodes HOG1 and SSK2 were affected to a much lesser extent, questioning our current understanding.


Marcus Krantz

University of Gothenburg

Lars-Göran Ottosson

University of Gothenburg

Doryaneh Ahmadpour

University of Gothenburg

Jonas Warringer

University of Gothenburg

Christian Waltermann

Bodil Nordlander

University of Gothenburg

Anders Blomberg

University of Gothenburg

Stefan Hohmann

University of Gothenburg

Hiroaki Kitano

9th International Conference on Systems Biology (ICSB2008) proceedings, 9th International Conference on Systems Biology (ICSB2008), August 22-28, Gothenburg

Subject Categories

Cell Biology

Biochemistry and Molecular Biology

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