CARS and Raman microscopy of Alzheimer's Brain Tissue
Conference contribution, 2010

Alzheimer’s disease (AD) is a progressive neurodegenerative disorder without cure, characterized by the presence of extracellular plaques surrounded by dystrophic neuritis . Fundamental understanding of the molecular processes involved is urgently needed in order to develop strategies to inhibit steps in the AD pathogenesis. Current understanding is that the senile plaques are composed of peptide fragments (Amyloid- β peptides) formed after the cleavage of the transmembrane protein amyloid protein precursor (APP) . This knowledge relies primarily on biochemical analysis of the plaques in cell extracts together with fluorescence microscopy of tissue samples exposed to harsh preparation procedures and labeling. Thus, it is disputed to which extent the observations reported reflect the true biochemistry and morphology of AD brain tissue. By the powerful combination of Raman microspectroscopy, Coherent Anti-Stokes Raman Scattering (CARS) and 2-photon fluorescence microscopy, we show that the molecular composition of AD plaques is more complex and consequently the mechanisms behind their formation.

Author

Annika Enejder

Chalmers, Chemical and Biological Engineering, Molecular Imaging

Fredrik Svedberg

Chalmers, Chemical and Biological Engineering, Molecular Imaging

Lena Nyberg

Chalmers, Chemical and Biological Engineering, Physical Chemistry

J. Y. Li

Lund University

AIP Conference Proceedings

0094-243X (ISSN) 1551-7616 (eISSN)

Vol. 1267 480-481

Subject Categories

Chemical Sciences

DOI

10.1063/1.3482627

ISBN

978-0-7354-0818-0

More information

Latest update

3/2/2018 9