Effects of PEGylation and acetylation of PAMAM dendrimers on DNA binding, cytotoxicity and in vitro transfection efficiency
Journal article, 2010

Poly(amidoamine) (PAMAM) dendrimers are promising multipotent gene delivery vectors, providing favourable DNA condensation properties also in combination with the possibility of conjugation of different targeting ligands to their surface. They have been used for transfection both in vitro and in vivo, but their application is currently somewhat limited due to inherent cytotoxicity. In this work we investigate how two types of surface modification, acetylation and PEGylation, affect the DNA binding characteristics, the cytotoxicity and the in vitro transfection efficiency of generation 4 and 5 PAMAM dendrimers. Particularly, we address how the morphology of DNA-dendrimer complexes, formed under low salt conditions, changes upon dilution in cell growth medium, an event that inevitably occurs before the complexes reach the cell surface in any transfection experiment. We find that acetylation and PEGylation essentially eliminates the inherent dendrimer cytotoxicity. However, the transfection efficiency of the modified dendrimers is lower than that of the corresponding unmodified dendrimers, which can be rationally understood by our observations that DNA is less condensed when complexed with these modified dendrimers. Although small DNA-dendrimer particles are formed, the availability for ethidium intercalation and nuclease degradation is significantly higher in the modified DNA-dendrimer complexes than in unmodified ones. Dilution in cell growth medium has a drastic effect on these electrostatically assembled complexes, resulting in increase in size and DNA availability. Our results strongly add to the notion that it is of importance to perform a biophysical characterization under conditions as close to the transfection situation as possible, to enable conclusions regarding structure- activity relations of gene delivery vectors.

gene delivery

polyplex

biocompatibility

DNA

characterization

Dendrimer

Author

Kristina Fant

Chalmers, Chemical and Biological Engineering, Physical Chemistry

E. K. Esbjorner

University of Cambridge

Alan Jenkins

University of Southampton

Martin Grossel

University of Southampton

Per Lincoln

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Bengt Nordén

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Molecular Pharmaceutics

1543-8384 (ISSN) 1543-8392 (eISSN)

Vol. 7 5 1734-1746

Subject Categories

Physical Chemistry

Chemical Sciences

Areas of Advance

Energy

Life Science Engineering (2010-2018)

Materials Science

DOI

10.1021/mp1001312

More information

Latest update

5/2/2018 7