Reusable hydroxyapatite nanocrystal sensors for protein adsorption
Paper in proceeding, 2010

The repeatability of the adsorption and removal of fibrinogen and fetal bovine serum on hydroxyapatite (HAp) nanocrystal sensors was investigated by Fourier transform infrared (FTIR) spectroscopy and quartz crystal microbalance with dissipation (QCM-D) monitoring technique. The HAp nanocrystals were coated on a gold-coated quartz sensor by electrophoretic deposition. Proteins adsorbed on the HAp sensors were removed by (i) ammonia/hydrogen peroxide mixture (APM), (ii) ultraviolet light (UV), (iii) UV/APM, (iv) APM/UV and (v) sodium dodecyl sulfate (SDS) treatments. FTIR spectra of the reused surfaces revealed that the APM and SDS treatments left peptide fragments or the proteins adsorbed on the surfaces, whereas the other methods successfully removed the proteins. The QCM-D measurements indicated that in the removal treatments, fibrinogen was slowly adsorbed in the first cycle because of the change in surface wettability revealed by contact angle measurements. The SDS treatment was not effective in removing proteins. The APM or UV treatment decreased the frequency shifts for the reused HAp sensors. The UV/APM treatment did not induce the frequency shifts but decreased the dissipation shifts. Therefore, we conclude that the APM/UV treatment is the most useful method for reproducing protein adsorption behavior on HAp sensors.

quartz crystal microbalance

protein adsorption

thin layer

hydroxyapatite sensor

reusability

Author

M. Tagaya

Tokyo Institute of Technology

National Institute for Materials Science (NIMS)

T. Ikoma

Tokyo Institute of Technology

National Institute for Materials Science (NIMS)

N. Hanagata

National Institute for Materials Science (NIMS)

Dinko Chakarov

Chalmers, Applied Physics, Chemical Physics

Bengt Herbert Kasemo

Chalmers, Applied Physics, Chemical Physics

J. Tanaka

Tokyo Institute of Technology

Science and Technology of Advanced Materials

1468-6996 (ISSN)

Vol. 11 4 045002

Subject Categories

Physical Sciences

DOI

10.1088/1468-6996/11/4/045002

More information

Latest update

4/5/2022 6