Effect of microwave radiation on permeability of liposomes. Evidence against non-thermal leakage
Journal article, 1994

The effect of 2.45 GHz microwave radiation on the permeability of unilamellar phosphatidylcholine liposomes has been studied. Leakage of 5(6)-calboxyfluorescein from the liposomes was measured using spectrofluorimetry after exposure to either microwaves or thermal heating for 5-20 min intervals. The exposure temperature, 37.6 +/- 0.5 degrees C, was well above the phase transition temperature of the lipid membrane. The microwave exposure did not result in any non-thermal increase in permeability above that produced by thermal heating. This study refutes the results reported by Saalman et al. [1] in which an increased liposome permeability due to microwave exposure was reported. The refined analysis in the present study shows that this increased liposome permeability was not a non-thermal microwave effect.

microwave radiation

liposome

nonthermal effect

phase-transition

fluorescence

5(6)-carboxyfluorescein

temperature

membrane permeability

erythrocytes

efflux

Author

Björn Bergqvist

Department of Microwave Technology

Lars Arvidsson

Department of Biochemistry and Biophysics

Eva Pettersson

Department of Biochemistry and Biophysics

Sheila Galt

Department of Microwave Technology

Elisabeth Saalman

Yngve Hamnerius

Department of Microwave Technology

Bengt Nordén

Department of Physical Chemistry

Biochimica et Biophysica Acta - General Subjects

0304-4165 (ISSN)

Vol. 1201 1 51-54

Subject Categories

Biochemistry and Molecular Biology

DOI

10.1016/0304-4165(94)90150-3

More information

Created

10/8/2017