Analysis of multiple scattering suppression using structured laser illumination planar imaging in scattering and fluorescing media
Journal article, 2011

The accuracy, precision and limitations of the imaging technique named Structured Laser Illumination Planar Imaging (SLIPI) have been investigated. SLIPI, which allows multiply scattered light to be diminished, has previously demonstrated improvements in image quality and contrast for spray imaging. In the current study the method is applied to a controlled confined environment consisting of a mixture of water and monodisperse polystyrene microspheres. Elastic scattering and fluorescence are studied and the results obtained when probing different particle concentrations and diameters conclusively show the advantages of SLIPI for imaging within moderately turbid media. Although the technique presents both good repeatability and agreement with the Beer-Lambert law, discrepancies in its performance were, however, discovered. Photons undergoing scattering without changing their incident trajectory cannot be discriminated and, owing to differences in scattering phase functions, probing larger particles reduces the suppression of multiply scattered light. However, in terms of visibility such behavior is beneficial as it allows denser media to be probed. It is further demonstrated that the suppression of diffuse light performs equally well regardless of whether photons propagate along the incident direction or towards the camera. In addition, this filtering process acts independently on the spatial distribution of the multiply scattered light but is limited by the finite dynamic range and unavoidable signal noise of the camera.

dense sprays

resolution

microscopy

Author

E. Kristensson

Lund University

L. Araneo

Polytechnic University of Milan

Edouard Berocal

Lund University

J. Manin

Polytechnic University of Valencia (UPV)

M. Richter

Lund University

M. Alden

Lund University

Mark Linne

Chalmers, Applied Mechanics, Combustion and Propulsion Systems

Optics Express

1094-4087 (ISSN) 10944087 (eISSN)

Vol. 19 14 13647-13663

Subject Categories

Mechanical Engineering

DOI

10.1364/OE.19.013647

More information

Latest update

3/29/2018