Interaction of Single Viruslike Particles with Vesicles Containing Glycosphingolipids
Journal article, 2011

Glycosphingolipids are involved in the first steps of virus-cell interaction, where they mediate specific recognition of the host cell membrane. We have employed total-internal-reflection fluorescence microscopy to explore the interaction kinetics between individual unlabeled noroviruslike particles, which are attached to a glycosphingolipid-containing lipid bilayer, and fluorescent vesicles containing different types and concentrations of glycosphingolipids. Under association equilibrium, the vesicle-binding rate is found to be kinetically limited, yielding information on the corresponding activation energy. The dissociation kinetics are logarithmic over a wide range of time. The latter is explained by the vesicle-size-related distribution of the dissociation activation energy. The biological, pharmaceutical, and diagnostic relevance of the study is briefly discussed.

mismatch discrimination

tracking

cells

virus

norwalk

noroviruses

infection pathway

entry

binding

bilayers

influenza-viruses

Author

Marta Bally

Chalmers, Applied Physics, Biological Physics

Anders Gunnarsson

Chalmers, Applied Physics, Biological Physics

L. Svensson

Göran Larson

University of Gothenburg

Vladimir Zhdanov

Chalmers, Applied Physics, Chemical Physics

Fredrik Höök

Chalmers, Applied Physics, Biological Physics

Physical Review Letters

0031-9007 (ISSN) 1079-7114 (eISSN)

Vol. 107 18

Subject Categories

Physical Chemistry

Physical Sciences

Clinical Laboratory Medicine

Nano Technology

Microbiology in the medical area

DOI

10.1103/PhysRevLett.107.188103

PubMed

22107678

More information

Created

10/8/2017