Mapping the interaction of Snf1 with TORC1 in Saccharomyces cerevisiae
Journal article, 2011

Nutrient sensing and coordination of metabolic pathways are crucial functions for all living cells, but details of the coordination under different environmental conditions remain elusive. We therefore undertook a systems biology approach to investigate the interactions between the Snf1 and the target of rapamycin complex 1 (TORC1) in Saccharomyces cerevisiae. We show that Snf1 regulates a much broader range of biological processes compared with TORC1 under both glucose-and ammonium-limited conditions. We also find that Snf1 has a role in upregulating the NADP(+)-dependent glutamate dehydrogenase (encoded by GDH3) under derepressing condition, and therefore may also have a role in ammonium assimilation and amino-acid biosynthesis, which can be considered as a convergence of Snf1 and TORC1 pathways. In addition to the accepted role of Snf1 in regulating fatty acid (FA) metabolism, we show that TORC1 also regulates FA metabolism, likely through modulating the peroxisome and beta-oxidation. Finally, we conclude that direct interactions between Snf1 and TORC1 pathways are unlikely under nutrient-limited conditions and propose that TORC1 is repressed in a manner that is independent of Snf1.

gene-expression

regulation

yeast snf1

nutrient sensing

carbon metabolism

signaling pathways

TORC1

glucose repression

kinetic-properties

transcriptional

nitrogen metabolism

Snf1

functional genomics

cell-growth control

chemical genomics

activated protein-kinase

Author

Jie Zhang

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

S. Vaga

Swiss Federal Institute of Technology in Zürich (ETH)

Pramote Chumnanpuen

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Rahul Kumar

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Goutham Vemuri

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

R. Aebersold

University of Zürich

Swiss Federal Institute of Technology in Zürich (ETH)

Jens B Nielsen

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Molecular Systems Biology

1744-4292 (ISSN)

Vol. 7 545

Subject Categories

Biochemistry and Molecular Biology

Roots

Basic sciences

Areas of Advance

Life Science Engineering (2010-2018)

DOI

10.1038/msb.2011.80

More information

Latest update

3/19/2018