Laminin Coating Promotes Calcium Phosphate Precipitation on Titanium Discs in vitro.
Journal article, 2011

Objectives: The objective of this study was to investigate the effect of a laminin coating on calcium phosphate precipitation on three potentially bioactive titanium surfaces in simulated body fluid. Material and Methods: Blasted titanium discs were prepared by alkali and heat treatment (AH), anodic oxidation (AO) or hydroxyapatite coating (HA) and subsequently coated with laminin. A laminin coated blasted surface (B) served as a positive control while a blasted non coated (B-) served as a negative control. Surface morphology was examined by Scanning Electron Microscopy (SEM). The analysis of the precipitated calcium and phosphorous was performed by Energy Dispersive X-ray Spectroscopy (EDX). Results: The thickness of the laminin coating was estimated at 26 Å by ellipsometry. Interferometry revealed that the coating process did not affect any of the tested topographical parameters on µm level when comparing B to B-. After 2 weeks of incubation in SBF, the alkali-heat treated discs displayed the highest calcium phosphate deposition and the B group showed higher levels of calcium phosphate than the B- group. Conclusions: Our results suggest that laminin may have the potential to be used as a coating agent in order to enhance the osseoinductive performance of biomaterial surfaces, with the protein molecules possibly functioning as nucleation centres for apatite formation. Nevertheless, in vivo studies are required in order to clarify the longevity of the coating and its performance in the complex biological environment.

osseointegration

calcium phosphates

laminin

titanium

dental implants

biomaterials

Author

Kostas Bougas

University of Gothenburg

Victoria Franke Stenport

University of Gothenburg

Pentti Tengvall

University of Gothenburg

Fredrik Currie

Ann Wennerberg

University of Gothenburg

Journal of Oral & Maxillofacial Research

2029-283X (ISSN)

Vol. 2 4 e5-

Subject Categories

Dentistry

DOI

10.5037/jomr.2011.2405

More information

Created

10/10/2017