Mechanical stimulation of fibroblasts in micro-channeled bacterial cellulose scaffolds enhances production of oriented collagen fibers
Journal article, 2012

Cellulose perforated by micro-channels (phi? similar to 500 mu m) has been investigated as a potential future scaffold material for meniscus implants. Scaffolds seeded with 3T6 fibroblasts were cultivated with mechanical stimulation in a compression bioreactor for enhanced collagen production. Constructs under dynamic compression at a frequency of 0.1 Hz and compression strain of 5% were compared to static cultures used as controls. The three-dimensional distributions of collagen fibers and fibroblasts in the cellulose scaffolds were studied under native, soft-matter conditions by combined second harmonic generation and coherent antiStokes Raman scattering microscopy, requiring no artificial sample preparation. Results showed that the micro-channels facilitated the alignment of cells and collagen fibers and that collagen production was enhanced by mechanical stimulation. Thus, cell-seeded, micro-channeled cellulose scaffolds provided guided tissue growth required to obtain an ultrastructure mimicking that of the meniscus.

collagen

micro-channels

SHG

structure

CARS microscopy

mechanical stimulation

bacterial cellulose

Author

Hector Martinez Avila

Chalmers, Chemical and Biological Engineering, Polymer Technology

Christian Brackmann

Chalmers, Chemical and Biological Engineering, Molecular Imaging

Annika Enejder

Chalmers, Chemical and Biological Engineering, Molecular Imaging

Paul Gatenholm

Chalmers, Chemical and Biological Engineering, Polymer Technology

Journal of Biomedical Materials Research - Part A

1549-3296 (ISSN)

Vol. 100A 4 948-957

Subject Categories

Chemical Sciences

DOI

10.1002/jbm.a.34035

More information

Created

10/7/2017