Neuronal cell growth on polymeric scaffolds studied by CARS microscopy
Paper in proceedings, 2012

For studies of neuronal cell integration and neurite outgrowth in polymeric scaffold materials as a future alternative for the treatment of damages in the neuronal system, we have developed a protocol employing CARS microscopy for imaging of neuronal networks. The benefits of CARS microscopy come here to their best use; (i) the overall three-dimensional (3D) arrangement of multiple cells and their neurites can be visualized without the need for chemical preparations or physical sectioning, potentially affecting the architecture of the soft, fragile scaffolds and (ii) details on the interaction between single cells and scaffold fibrils can be investigated by close-up images at sub-micron resolution. The establishment of biologically more relevant 3D neuronal networks in a soft hydrogel composed of native Extra Cellular Matrix (ECM) components was compared with conventional two-dimensional networks grown on a stiff substrate. Images of cells in the hydrogel scaffold reveal significantly different networking characteristics compared to the 2D networks, raising the question whether the functionality of neurons grown as layers in conventional cultivation dishes represents that of neurons in the central and peripheral nervous systems.

Polymeric scaffold

Single cells

Multiple cells

Hydrogel scaffolds

Neurites

Two-dimensional networks

Extracellular matrices

Neuronal cell

Chemical preparation

Submicron

Neurite outgrowth

Neuronal systems

Peripheral nervous system

Neuronal networks

Author

Annika Enejder

Chalmers, Chemical and Biological Engineering, Molecular Imaging

Helen Fink

Chalmers, Chemical and Biological Engineering, Molecular Imaging

Hans-Georg Kuhn

University of Gothenburg

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

16057422 (ISSN)

Vol. 8226 82261W

Subject Categories

Biochemistry and Molecular Biology

DOI

10.1117/12.910134

ISBN

978-08-19-48869-5

More information

Created

10/7/2017