Radial Sizing of Lipid Nanotubes Using Membrane Displacement Analysis
Journal article, 2012
We report a novel method for the measurement of lipid nanotube radii. Membrane translocation is monitored between two nanotube-connected vesicles, during the expansion of a receiving vesicle, by observing a photobleached region of the nanotube. We elucidate nanotube radii, extracted from SPE vesicles, enabling quantification of membrane composition and lamellarity. Variances of nanotube radii were measured, showing a growth of 40-56 nm, upon increasing cholesterol content from 0 to 20%.
membrane displacement
liposomes
target-cells
transmission
intracellular trafficking
cholesterol
depletion
transport
cholesterol
vesicle
microscopy
proteins
plasma-membrane
Lipid nanotube
Author
Natalia Stepanyants
Chalmers, Chemical and Biological Engineering, Physical Chemistry
Gavin Jeffries
Chalmers, Chemical and Biological Engineering, Physical Chemistry
Owe Orwar
Chalmers, Chemical and Biological Engineering, Physical Chemistry
Aldo Jesorka
Chalmers, Chemical and Biological Engineering, Physical Chemistry
Nano Letters
1530-6984 (ISSN) 1530-6992 (eISSN)
Vol. 12 3 1372-1378Subject Categories
Chemical Sciences
DOI
10.1021/nl203983e