Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids
Journal article, 2012

Förster resonance energy transfer (FRET) is a powerful tool for monitoring molecular distances and interactions at the nanoscale level. The strong dependence of transfer efficiency on probe separation makes FRET perfectly suited for on/off experiments. To use FRET to obtain quantitative distances and three-dimensional structures, however, is more challenging. This review summarises recent studies and technological advances that have improved FRET as a quantitative molecular ruler in nucleic acid systems, both at the ensemble and at the single-molecule levels.

probability-distribution

DNA structures

double-stranded dna

multiparameter fluorescence detection

alternating-laser

photon distribution analysis

fluorescence spectroscopy

nano-positioning system

excitation

resonance energy-transfer

photoinduced electron-transfer

fluorescent probes

nucleic acids

single-molecule fret

analysis

rna-polymerase-ii

FRET

Author

S. Preus

University of Copenhagen

Marcus Wilhelmsson

Chalmers, Chemical and Biological Engineering, Physical Chemistry

ChemBioChem

1439-4227 (ISSN) 1439-7633 (eISSN)

Vol. 13 14 1990-2001

Areas of Advance

Nanoscience and Nanotechnology

Life Science Engineering

Subject Categories

Physical Chemistry

Biochemistry and Molecular Biology

Biophysics

Chemical Sciences

DOI

10.1002/cbic.201200400

More information

Latest update

5/8/2018 1