Kinetics of the enzyme-vesicle interaction including the formation of rafts and membrane strain
Journal article, 2012

In cells, an appreciable part of enzymes is associated with lipid membranes. Academic experimental studies of the function of membrane enzymes (e.g., PLA(2) representing a prototype for interfacial enzymology) are often focused on the enzyme-vesicle interaction or, more specifically, on conversion of lipid forming the external leaflet of the vesicle membrane. The corresponding kinetics are complicated by many factors inherent to the interfacial physics and chemistry. The understanding of the relative role of such factors and how they should be quantitatively described is still limited. Here, we present the mean-field kinetic equations, taking the formation of rafts in the membrane and the product-induced membrane strain into account, and analyze various scenarios of lipid conversion. In particular, we scrutinize the conditions when the kinetics may exhibit a transition from a relatively long latency period to a steady-state regime with fast nearly constant reaction rate. Specifically, we discuss the likely role of the pore formation in the external lipid layer in this transition. The latter effect may be caused by the product-induced tensile strain in this layer.

Enzymatic reactions

elasticity

phase

surfaces

Hydrolysis of lipids

interfacial activation

catalysis

lipid-bilayers

motions

Lipid membranes

mechanotransduction

phospholipase a(2) hydrolysis

Mean-field kinetic models

Author

Vladimir Zhdanov

Chalmers, Applied Physics, Chemical Physics

Fredrik Höök

Chalmers, Applied Physics, Biological Physics

Biophysical Chemistry

0301-4622 (ISSN)

Vol. 170 17-24

Subject Categories

Condensed Matter Physics

DOI

10.1016/j.bpc.2012.06.004

More information

Created

10/7/2017