Carbon-Ring Microelectrode Arrays for Electrochemical Imaging of Single Cell Exocytosis: Fabrication and Characterization
Journal article, 2012

Fabrication of carbon microelectrode arrays, with up to 15 electrodes in total tips as small as 10-50 mu m, is presented. The support structures of microelectrodes were obtained by pulling multiple quartz capillaries together to form hollow capillary arrays before carbon deposition. Carbon ring microelectrodes were deposited by pyrolysis of acetylene in the lumen of these quartz capillary arrays. Each carbon deposited array tip was filled with epoxy, followed by beveling of the tip of the array to form a deposited carbon-ring microelectrode array (CRMA). Both the number of the microelectrodes in the array and the tip size are independently tunable. These CRMAs have been characterized using scanning electron microscopy, energy dispersive X-ray spectroscopy, and electrogenerated chemiluminescence. Additionally, the electrochemical properties were investigated with steady-state voltammetry. In order to demonstrate the utility of these fabricated microelectrodes in neurochemistry, CRMAs containing eight microring electrodes were used for electrochemical monitoring of exocytotic events from single PC12 cells. Subcellular temporal heterogeneities in exocytosis (i.e. cold spots vs hot spots) were successfully detected with the CRMAs.

Author

Y. Q. Lin

University of Gothenburg

R. Trouillon

University of Gothenburg

M. I. Svensson

University of Gothenburg

Jacqueline Keighron

Chalmers, Chemical and Biological Engineering, Analytical Chemistry

Ann-Sofie Cans

Chalmers, Chemical and Biological Engineering, Analytical Chemistry

Andrew Ewing

Chalmers, Chemical and Biological Engineering, Analytical Chemistry

Analytical Chemistry

0003-2700 (ISSN) 1520-6882 (eISSN)

Vol. 84 6 2949-2954

Subject Categories

Chemical Sciences

DOI

10.1021/ac3000368

More information

Latest update

2/21/2018