Reconstruction of Biological Networks for Integrative Analysis
Doctoral thesis, 2014

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transcriptional regulation

: Biological networks

integrative analysis

protein secretion

genome-scale metabolic model

Author

Tobias Österlund

Chalmers, Chemical and Biological Engineering, Life Sciences

Included papers

Popular science description

English

Biological systems are very complex and can involve thousands of components such as genes, proteins and small molecules. Systems biology uses mathematical approaches to model the system based on the involved components and their interactions in order to better understand how a cell respond to a change of environment or as a function of a disease. Biological network reconstructions can help to understand the complexity of the cell by studying the system as a whole rather than studying individual components. The yeast Saccharomyces cerevisiae can be used as a host to produce other products, e.g. biofuels and proteins such as amylase or insulin. Understanding the biology of yeast on the systems level can help to produce more products with higher yields. In order to get an increased understanding of the biological system we need more comprehensive network reconstructions that cover many processes and pathways in the cell. In my thesis work I have expanded previous biological network reconstructions and created a new, more comprehensive genome-scale model of the yeast metabolism, called iTO977. Further we have reconstructed a model of the protein secretion machinery for yeast and Aspergillus oryzae. I have also investigated the topology and organization of the yeast transcriptional regulatory network. The biological network reconstructions were also used as a scaffold for integrative analysis in order to understand how the cell behaves in different conditions.

Categorizing

Areas of Advance

Life Science Engineering (2010-2018)

Subject Categories (SSIF 2011)

Bioinformatics and Systems Biology

Identifiers

ISBN

978-91-7385-960-8

Other

Series

Doktorsavhandlingar vid Chalmers tekniska högskola. Ny serie: 3641

Public defence

2014-02-14 14:30

KA

Opponent: Kiran Patil, EMBL, Heidelberg, Germany

More information

Created

10/8/2017