Structure and composition of biofilm communities in a moving bed biofilm reactor for nitritation-anammox at low temperatures.
Journal article, 2014

It is a challenge to apply anaerobic ammonium oxidation (anammox) for nitrogen removal from wastewater at low temperatures. Maintenance of anammox- and aerobic ammonia oxidizing bacteria (AOB) and suppression of nitrite oxidizing bacteria (NOB) are key issues. In this work, a nitritation-anammox moving bed biofilm pilot reactor was operated at 19-10°C for 300d. Nitrogen removal was decreasing, but stable, at 19-13°C. At 10°C removal became unstable. Quantitative PCR, fluorescence in situ hybridization and gene sequencing showed that no major microbial community changes were observed with decreased temperature. Anammox bacteria dominated the biofilm (0.9-1.2×10(14) 16S rRNA copies m(-2)). Most anammox bacteria were similar to Brocadia sp. 40, but another smaller Brocadia population was present near the biofilm-water interface, where also the AOB community (Nitrosomonas) was concentrated in thin layers (1.8-5.3×10(12) amoA copies m(-2)). NOB (Nitrobacter, Nitrospira) were always present at low concentrations (<1.3×10(11) 16S rRNA copies m(-2)).

Author

Frank Persson

Chalmers, Civil and Environmental Engineering, Water Environment Technology

Razia Sultana

Royal Institute of Technology (KTH)

Marco Suarez

University of Gothenburg

Malte Hermansson

University of Gothenburg

Elzbieta Plaza

Royal Institute of Technology (KTH)

Britt-Marie Wilen

Chalmers, Civil and Environmental Engineering, Water Environment Technology

Bioresource technology

1873-2976 (eISSN)

Vol. 154 267-273

Driving Forces

Sustainable development

Areas of Advance

Building Futures (2010-2018)

Subject Categories

Water Engineering

Other Environmental Engineering

Microbiology

DOI

10.1016/j.biortech.2013.12.062

PubMed

24412479

More information

Latest update

2/26/2018