Bacteria-material surface interactions: methodological development for the assessment of implant surface induced antibacterial effects.
Journal article, 2015

The choice of material for implanted prostheses is of great importance concerning bacterial colonization and biofilm formation. Consequently, methods to investigate bacterial behavior are needed in order to develop new infection resistant surfaces. In this study, different methodological setups were used to evaluate the antimicrobial effect of photocatalytic titanium oxide and silver surfaces. Biofilm formation and eradication under static and dynamic culture conditions were studied with the use of the following analytical techniques: viable colony-forming unit (CFU) counting, imprinting, fluorescence, and bioluminescence. The present study demonstrates that different methods are needed in order to evaluate the prophylactic and treatment effects on planktonic and biofilm bacteria and to assess the antimicrobial effect of different surface treatments/coatings. Choosing the right antibacterial testing model for the specific application is also of great importance. Both in situ approaches and indirect methods provide valuable complementary information. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2014.


Magdalena Zaborowska

University of Gothenburg

Ken Welch

Rickard Brånemark

Poroshat Khalilpour

Håkan Engqvist

Peter Thomsen

University of Gothenburg

Margarita Trobos

University of Gothenburg

Journal of biomedical materials research. Part B, Applied biomaterials

1552-4981 (ISSN)

Vol. 103 1 179-187

Subject Categories







More information